Views: 0 Author: Site Editor Publish Time: 2022-11-04 Origin: Site
Sodium cyclamate, whose chemical name is sodium cyclohexylaminosulfonate, is a common additive in food production, its sweetness is 30-40 times that of sucrose, often white crystals or white crystalline powder, and its characteristics are odorless, sweet, easily soluble in water, insoluble in ethanol, insoluble in chloroform and ether, slightly decomposed under acidic conditions, stable under alkaline conditions.
Here is the content list:
Thin layer chromatography
Atomic absorption spectrophotometry
Turbidity method
Mass spectrometry method
The thin layer chromatography method starts with making a thin layer chromatography plate, then the extracted sample is spotted on the plate, and through its interaction with the chromogenic agent, the sample containing sodium cyclamate develops color spots, this method does not require any instrument support and the detection cost is low.
The spectrophotometric method uses the reaction of sodium cyclamate with a certain reagent to produce a colored compound, and the absorbance of this substance at a certain wavelength and its concentration follow Lambert's law, which is used for quantitative analysis. This method does not require large instruments because of the simple instrumentation used and the ease of operation, and it is simple and inexpensive to operate.
Sodium cyclamate reacts with barium chloride and sodium nitrite in an acidic environment to form a milky white precipitate of barium cyclamate, which becomes a characteristic reaction of sodium cyclamate. The method is easy to operate, highly sensitive, simple to use, and easy to carry, so it has become one of the fast detection methods for sodium cyclamate.
1. Gas chromatography-tandem mass spectrometry
Gas chromatography-mass spectrometry is a rapid detection and analysis method developed in recent years, in which the sample processing is simple, qualitative and accurate, high precision, and suitable for the qualitative and quantitative analysis and determination of sodium cyclamate in food.
2. Liquid chromatography-tandem mass spectrometry
Because liquid chromatography-tandem mass spectrometry does not require derivatization and can determine a variety of food additives, including sodium cyclamate, it has become a new choice for multi-component separation and identification. Compared with liquid chromatography, liquid-liquid mass spectrometry utilizes the strong separation ability of high-performance liquid chromatography and the characteristics of accurate characterization, high selectivity, and high sensitivity of mass spectrometry, while reducing matrix interference and lower detection limits, making it a better choice for the detection of sodium cyclamate and the discrimination of false positives.
3. Ion chromatography-tandem mass spectrometry
Solid-phase extraction column is used for online enrichment of acidified water samples, and then separated by the ion chromatography separation principle, and finally unified with multiple reactions to monitor the negative ion mode for the collection and detection of a variety of artificial sweeteners. The method is simple, with low interference and low detection limits, and can detect trace or trace amounts of sodium cyclamate in samples.
4. Liquid chromatography/quadrupole-time-of-flight mass spectrometry
Time-of-flight mass spectrometry is a high-resolution mass spectrometry, which is the most effective method for the rapid determination of multiple components in the future. This method can quickly screen a variety of additives including sodium cyclamate, providing a time-saving and convenient method for food safety testing while accurately qualifying and quantifying, as well as providing protection against unexpected situations.
The above is the relevant content about the detection of sodium cyclamate. If you are interested in sodium cyclamate you can contact us, our website is www.jysunway.com.I look forward to your arrival very much and hope to cooperate with you.
