Views: 0 Author: Site Editor Publish Time: 2022-12-08 Origin: Site
As we all know, the level of glucose in body fluids is an important indicator of human health, especially blood glucose monitoring for diabetics. Sudden increases and decreases in glucose concentrations signal an abnormality. Therefore, the detection of glucose has become more and more important. The following are several methods of glucose detection.
The following is a list of contents:
Microdialysis
Spectrophotometry
Optical rotation method
Biosensor method
Gas chromatography
The detection method of glucose concentration in tissue fluid uses microdialysis technology to detect the glucose concentration in the tissue fluid. The microdialysis probe inserted into the subcutaneous tissue is perfused under non-equilibrium conditions. The permeable membrane enters the dialysis tube and is continuously carried out by the perfusate flowing in the dialysis tube. Finally, the outflow dialysate is detected to obtain the concentration of glucose in the tissue fluid. This detection method has the advantages of strong continuity, small sampling volume, and small tissue trauma.
Determination of glucose is an important test item in clinical chemistry. Molybdenum heteropolyacid is an important color developing agent in photometric analysis. It is mainly used for the determination of silicon, phosphorus, and other substances. Scientists have found that the reaction of glucose and phosphomolybdic heteropoly acid can produce colored products. Its maximum absorption wavelength is 690nm, and the absorbance is linearly related to the mass concentration of glucose. This method has been used to determine the glucose content in glucose injection.
In the investigation of the linear relationship between the glucose concentration and the optical rotation value, an appropriate amount of pure glucose dried to a constant weight at 98-100 °C was accurately weighed and dissolved in water, and diluted. The results show that when the glucose concentration is within a reasonable range, there is a good linear relationship. "Chinese Pharmacopoeia" uses the optical rotation method for the determination of the glucose content in the glucose APIs and injections. Scientists studied the optical rotation method for the determination of glucose content in compound Danshen injection and investigated the color of the injection and the effect of adding ammonia test solution during dilution on the determination. There is a good linear relationship between glucose concentration and optical rotation value. The research shows that the optical rotation of glucosamine hydrochloride has a good linear relationship with the concentration within a certain concentration range. The determination of amino-glucose hydrochloride by optical polarimetry has good sample recovery rates at low, medium, and high concentration levels, indicating that the sample addition recovery rate of preparation excipients does not interfere with the determination when this method is used to determine the content, which fully meets the experimental requirements.
The working principle of the glucose biosensor is as follows: glucose first generates hydrogen peroxide under the catalytic action, and under the catalysis of peroxidase, the reduced state dielectric is oxidized to an oxidized state dielectric, which is reduced by reduction at a lower operating voltage. There is a linear relationship between the current and the measured glucose concentration. The corresponding glucose concentration can be obtained by detecting the reduction current.
When gas chromatography is used to analyze polyhydroxy compounds such as glucose, due to the high polarity and high boiling point of polyhydroxy compounds, direct injection analysis cannot be carried out. The trimethylsilyl ether derivatives were then analyzed by gas chromatography. Scientists and others used bistrimethylsilylacetamide and trimethylchlorosilane as silicon etherification reagents and carried out quantitative analysis of the dilute aqueous solution of glucose and mannitol by gas chromatography. The results were accurate and reliable.
Diabetes is a disease that seriously endangers human health. Continuous blood glucose testing and glucose testing are of great clinical significance. At present, according to the degree of damage to the human body by the detection method, the dynamic and continuous monitoring of the glucose concentration in the human body can be realized. It has important application value and commercial prospects. After reading the above content, if you are interested in glucose, you can check our company's website www.jysunway.com, we look forward to your arrival.
